Chapter 5

Enzyme Action

Enzymes are protein catalysts that lower activation energy and act on specific substrates via the lock-and-key model.

What are enzymes?

Enzymes are biological catalysts made of protein. They speed up the rate of chemical reactions in living organisms but are not used up, so a small amount can be used again and again.

Key idea

An enzyme lowers the activation energy needed for a reaction, so the reaction happens faster at body temperature. Enzyme + substrate → enzyme-substrate complex → enzyme + product(s).

The lock-and-key model

Each enzyme has a region called the active site with a specific shape. Only a substrate with a complementary shape can fit into it, like a key fitting a lock. This is why enzymes are specific — one enzyme usually works on only one substrate. The substrate binds to form an enzyme-substrate complex; the reaction takes place, and the products are released, leaving the enzyme unchanged.

Some named enzymes

  • Amylase breaks down starch into maltose (sugar).
  • Protease breaks down protein into amino acids.
  • Lipase breaks down fats into fatty acids and glycerol.
  • Catalase breaks down hydrogen peroxide into water and oxygen.

Enzymes control almost every reaction in a cell, including those that build large molecules as well as those that break them down. Because only a matching substrate fits the active site, a cell can control its many reactions separately by making different enzymes. A tiny amount of enzyme can catalyse a very large number of reactions, one after another, since the enzyme is released unchanged each time and is free to bind another substrate molecule.

Remember

  • Enzymes are proteins and catalysts — not used up in the reaction.
  • Only a matching substrate fits the active site (specificity).
  • Enzymes lower activation energy; they do not raise it.

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